Gene Expression Changes Key To Revealing Mechanism That Leads To Addiction

Dr. Judith A. Potashkin, Ph.D. and her colleagues at Rosalind Franklin University of Medicine and Science recently completed a study investigating one of the changes in gene expression that occurs when individuals take addictive drugs. The findings of this study will be published in the September 5 issue of the online, open-access journal PLoS ONE.



Dr. Potashkin, Associate Professor and Vice Chair of the Department of Cellular and Molecular Pharmacology, is an expert in gene expression. She commented, "Addiction is a brain disorder that manifests itself by repetitive behaviors despite negative consequences. Currently, there is an abundance of information known about the cellular and behavioral changes that occur during addiction, but little is understood concerning the changes that occur at the molecular level with regards to gene expression. Understanding the changes that occur between transcription and protein synthesis is key to revealing the mechanism that leads to addiction."



Dr. Potashkin's studies focus on how the primary RNA transcript is processed by splicing to produce a mature transcript. The fidelity of splicing must be maintained since errors may lead to the development of disease. One immediate and prominent alteration that occurs with administration of amphetamine or cocaine is the accumulation in one region of the brain of very stable truncated isoform of the transcription factor FosB termed DFosB that is produced by alternative splicing of the transcript. DFosB mediates some of the neural and behavioral modifications that occur with drug addiction. The results from the study identified a splicing factor, polypyrimidine tract binding protein, as a key factor in regulating the switch in splicing that result in the truncated form of FosB being produc ed instead of the less stable full-length protein. The study also provided clues about the signaling pathway that is activated that leads to splicing regulation. This information provides several potential therapeutic targets for drug addiction.







Dr. Potashkin has been studying gene expression for the past 22 years. This research was funded by the National Institute of Drug Addiction, a division of the National Institutes of Health. Dr. Potashkin is also funded by the Department of Defense.



Rosalind Franklin University of Medicine and Science educates medical doctors, health professionals and biomedical scientists in a personalized atmosphere. The University is located at 3333 Green Bay Road, North Chicago, IL 60064, and encompasses Chicago Medical School, College of Health Professions, Dr. William M. Scholl College of Podiatric Medicine, and School of Graduate and Postdoctoral Studies. Visit at rosalindfranklin and lifeindiscovery



Contact:



Priscilla Khoury, MS, FAHP

Vice President for Institutional Advancement

rosalindfranklin



Citation: Marinescu V, Loomis PA, Ehmann S, Beales M, Potashkin JA (2007) Regulation of Retention of FosB Intron 4 by PTB. PLoS ONE 2(9): e828.doi:10.1371/journal.pone.0000828



LINK TO THE PUBLISHED ARTICLE: plosone/doi/pone.0000828



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Contact: Priscilla Khoury

Public Library of Science